cellsens scoring software  (Olympus)

Journal: The American journal of pathology

Article Title: Urothelium-Specific Expression of Mutationally Activated Pik3ca Initiates Early Lesions of Noninvasive Bladder Cancer.

doi: 10.1016/j.ajpath.2023.07.001

Figure Lengend Snippet: Figure 2 Upk2-Cre/Pik3caH1047R mice exhibit increased urothelial thickness. A: Representative hematoxylin and eosin images of control and Upk2-Cre/Pik3caH1047R mice with one or two alleles of mutant Pik3ca aged for 6 and 12 months. Upk2- Cre/Pik3caH1047R mice develop thicker urothelium after 6 and 12 months of aging. The thickness of the urothelium is indicated using arrows. B: Dot plots illustrating urothelial thickness (mm) of Upk2-Cre/Pik3caH1047R mice at 6 and 12 months of age. At 6 months, urothelial thickness is signifi- cantly increased in mice with one and two alleles of Pik3caH1047R compared with controls (P Z 0.0001 and P < 0.0001, respectively; Tukey multiple-comparisons test). Urothelial thickness is also significantly increased in mice aged 12 months with one and two alleles of Pik3caH1047R

Article Snippet: Urothelial Thickness and Histologic Scoring Urothelial thickness was measured using cellSens Entry 1.13 imaging software (Olympus, Center Valley, PA) on hematoxylin and eosin sections.

Techniques: Control, Mutagenesis

Journal: The American journal of pathology

Article Title: Urothelium-Specific Expression of Mutationally Activated Pik3ca Initiates Early Lesions of Noninvasive Bladder Cancer.

doi: 10.1016/j.ajpath.2023.07.001

Figure Lengend Snippet: Figure 3 Luminal differentiation markers forkhead box A1 (Foxa1) and peroxisome proliferator-activated receptor g (Pparg) are highly expressed in Upk2-Cre/Pik3caH1047R mouse urothelium. Immunohistochemical staining of luminal (Foxa1 and Pparg) and basal [keratin (Krt) 5/6 and Krt14] markers of urothelial differentia- tion in Upk2-Cre/Pik3caH1047R mice aged 6 and 12 months. High expression of luminal markers Foxa1 and Pparg is maintained in the urothelium of mice with one and two alleles of Pik3caH1047R. In addi- tion, basal markers Krt5/6 and Krt14 remain low in expression. Scale bars Z 20 mm.

Article Snippet: Urothelial Thickness and Histologic Scoring Urothelial thickness was measured using cellSens Entry 1.13 imaging software (Olympus, Center Valley, PA) on hematoxylin and eosin sections.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: The American journal of pathology

Article Title: Urothelium-Specific Expression of Mutationally Activated Pik3ca Initiates Early Lesions of Noninvasive Bladder Cancer.

doi: 10.1016/j.ajpath.2023.07.001

Figure Lengend Snippet: Figure 4 Upk2-Cre/Pik3caH1047R mice do not show significant differ- ences in susceptibility to N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN), as measured by urothelial thickness. Six-montheold control and mutant mice with two alleles of Pik3caH1047R were given 0.01% BBN in their drinking water for 10 weeks. These mice were then compared with the 6-montheold mice previously assessed, which were not exposed to BBN. A: Hematoxylin and eosin (H&E) staining shows slightly thicker urothelium of mutant mice with similar morphology to control after exposure to BBN. B: Urothelial thickness (mm) measurements show no significant difference driven by Pik3ca mutation in combination with BBN exposure [P Z 0.3941; two-way analysis of variance (ANOVA) with interaction]. The two-way ANOVA model was then refit without the interaction term. The marginal test for BBN is not significant (P Z 0.2097); however, the marginal test for Pik3caH1047R

Article Snippet: Urothelial Thickness and Histologic Scoring Urothelial thickness was measured using cellSens Entry 1.13 imaging software (Olympus, Center Valley, PA) on hematoxylin and eosin sections.

Techniques: Control, Mutagenesis, Staining